NP40 Cell Lysis Buffer (2X), 7.4 pH, 125mL
UZS 852291
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Manufactured in an ISO 9001 and 13485 certified facility in the USA, our buffers ensure the highest quality for your research needs.
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Информация о продукте
| Container Type | Bottle |
| Item Form | Liquid |
| Item Weight | 4 ounces (113.4 grams) |
| Volume | 125 ml |
| Brand Name | eCon Lab Supply |
| Manufacturer | Ciro Mfg |
| Included Components | Bottle |
| Part Number | 23.105.113 |
| Brand | eCon Lab Supply |
| Item Volume | 125 ml |
| Package Information | Bottle |
Who Should Buy?
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Biotechnology Researchers
Ideal for researchers conducting experiments in cell biology requiring cell lysis for protein extraction and analysis.
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Molecular Biologists
Beneficial for molecular biologists isolating nucleic acids from cell samples through efficient lysis techniques.
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Academic Laboratories
Perfect for educational settings where practical experimentation with cell lysates is part of the curriculum.
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Home Users
Not suitable for casual home experiments due to specialized nature and required safety protocols of the buffer.
ОПИСАНИЕ ТОВАРА
NP40 Cell Lysis Buffer (2X), 7.4 pH, 125mL
Вопросы и ответы клиентов
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вопрос:
What is NP40 Cell Lysis Buffer 2X used for?
отвечать: NP40 Cell Lysis Buffer 2X is primarily used to lyse cells, making it an essential reagent in molecular biology and biochemistry. It helps to disrupt cell membranes, allowing researchers to extract proteins, DNA, RNA, and other cellular components. This buffer is particularly useful for studies involving protein expression, purification, and analysis in both clinical and research settings. -
вопрос:
How should NP40 Cell Lysis Buffer 2X be prepared for use?
отвечать: The NP40 Cell Lysis Buffer 2X is typically supplied as a concentrated solution. To prepare it for use, simply dilute it with an appropriate buffer or distilled water as recommended in your experimental protocol. This allows for optimal lysis while ensuring compatibility with various downstream applications such as Western blotting, ELISA, or PCR analysis. -
вопрос:
Can NP40 Cell Lysis Buffer be used for various cell types?
отвечать: Yes, NP40 Cell Lysis Buffer is versatile and can be used on multiple cell types, including mammalian, bacterial, and yeast cells. However, keep in mind that each cell type may have specific requirements for lysis conditions, such as incubation time and temperature. For best results, refer to the protocols designed for the specific cells you are working with. -
вопрос:
Is NP40 Cell Lysis Buffer compatible with downstream applications?
отвечать: Absolutely, NP40 Cell Lysis Buffer is designed to be compatible with a wide range of downstream applications such as protein analysis, immunoprecipitation, and nucleic acid extraction. It provides a balance of cell disruption while preserving the integrity of biomolecules, making it ideal for various experimental protocols. -
вопрос:
What is the pH of NP40 Cell Lysis Buffer 2X, and why is it important?
отвечать: The NP40 Cell Lysis Buffer comes at a pH of 7.4, which is critical for maintaining the stability of biomolecules during the lysis process. This neutral pH promotes optimal enzyme activity and prevents denaturation of proteins, ensuring that the extracted components are functional and suitable for analysis. -
вопрос:
How long can I store NP40 Cell Lysis Buffer 2X?
отвечать: NP40 Cell Lysis Buffer 2X can generally be stored at 4°C for short-term use, but for long-term storage, it is often recommended to keep it at -20°C. Proper storage conditions help maintain the buffer's efficacy and stability, allowing for reliable results in your experiments over time. -
вопрос:
Is NP40 Cell Lysis Buffer 2X toxic or hazardous?
отвечать: NP40 Cell Lysis Buffer contains detergents that can be hazardous if not handled properly. Therefore, it is important to use personal protective equipment, such as gloves and goggles, while working with the buffer. Adhering to safety protocols can help ensure a safe working environment during your experiments. -
вопрос:
Can I use NP40 Cell Lysis Buffer 2X for both protein and nucleic acid extraction?
отвечать: Yes, NP40 Cell Lysis Buffer 2X is suitable for both protein and nucleic acid extraction. Its formulation allows for efficient cell lysis while preserving the integrity of proteins and nucleic acids, which can be particularly beneficial in experiments where both types of biomolecules are required for analysis or further study. -
вопрос:
What cell lysis protocols can be used with NP40 Cell Lysis Buffer?
отвечать: Protocols for cell lysis with NP40 Cell Lysis Buffer can vary depending on the type of cells being lysed. Common approaches include sonication, freeze-thaw cycles, or enzymatic digestion to enhance lysis efficiency. It’s essential to refer to specific guidelines based on your experimental needs to achieve optimal results. -
вопрос:
Where can I buy NP40 Cell Lysis Buffer 2X, 7.4 pH, 125mL in Uzbekistan?
отвечать: You can purchase NP40 Cell Lysis Buffer 2X, 7.4 pH, 125mL from Ubuy, which offers a reliable source for various laboratory reagents and products. Ubuy provides a convenient platform to find this essential buffer along with other scientific supplies tailored to your needs.
eCon Lab Supply Buffers Editorial Review
Customer Reviews & Ratings
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5 звезда
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UZS 852291
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Особенности и преимущества
- Comprehensive range of biological buffers for various applications.
- Ideal for protein research, molecular biology, and characterization.
- Produced in a certified facility adhering to stringent quality control.
- NP40 Cell Lysis Buffer is perfect for Antibody Bead Immunoassay and ELISA.
- Designed for cell extract preparation and immunoprecipitation wash.
- Soluble in water for easy use in laboratory settings.
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