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EMS 22146 Triton X-100, 0.08% Aqueous Solution, 500 mL
UZS 1363300
Price Details
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Product Details
| Item Weight | 1.1 lbs (500 grams) |
Who Should Buy?
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Biotechnology Labs
Ideal for biotechnologists needing a gentle surfactant for cell lysis and protein extraction without denaturing proteins.
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Research Institutions
Useful for researchers in molecular biology who require a reliable non-ionic detergent in aqueous solutions.
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Cell Culture Applications
Beneficial for cell culture applications where a mild cleaning agent is needed to maintain cellular integrity.
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Heavy Cleaning Applications
Not suitable for applications requiring a strong detergent for heavy-duty cleaning tasks or grease removal.
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Food Industry Use
Inappropriate for food processing or direct contact with food items due to lack of food-grade certification.
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Industrial Manufacturing
Not ideal for large-scale industrial applications requiring robust surfactants designed for high-volume production environments.
Product Description
EMS 22146 Triton X-100, 0.08% Aqueous Solution, 500 mL
Customer Questions & Answers
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Question:
What is EMS 22146 Triton X-100, 0.08% Aqueous Solution used for?
Answer: EMS 22146 Triton X-100, 0.08% Aqueous Solution is primarily used as a non-ionic surfactant in various laboratory applications. It helps to improve the solubility of hydrophobic compounds, making it an essential reagent in biochemistry and molecular biology experiments. By reducing surface tension, Triton X-100 is often used in cell lysis protocols, protein purification, and immunoassays. This allows researchers to effectively extract proteins from cells or tissues for study, streamlining the research process. -
Question:
How do I properly store EMS 22146 Triton X-100?
Answer: To maintain its efficacy, EMS 22146 Triton X-100 should be stored in a cool, dry place away from direct sunlight. It's ideal to keep the solution at room temperature, ensuring that the container is tightly sealed to prevent contamination or evaporation. Proper storage helps preserve the integrity of the surfactant properties, ensuring reliable results during experimental use. Always check the label for any specific storage instructions to ensure optimal performance. -
Question:
Is EMS 22146 Triton X-100 compatible with all cell types?
Answer: EMS 22146 Triton X-100 is compatible with many cell types but may not be suitable for all. Generally, it works well with mammalian cells, yeast, and some bacterial cells. However, for sensitive cell types, it is vital to assess its effects on cell viability and functionality, as concentrations or exposure duration may vary in different experiments. Researchers should conduct preliminary tests to ensure that Triton X-100 does not adversely affect their specific cell lines, thereby optimizing experimental outcomes. -
Question:
Can EMS 22146 Triton X-100 be used for protein extraction?
Answer: Yes, EMS 22146 Triton X-100 is widely used for protein extraction. The non-ionic nature of the surfactant helps solubilize membrane proteins, allowing for efficient extraction from cell membranes without denaturing them. Researchers commonly employ Triton X-100 in lysis buffers to extract proteins for various applications including Western blotting and enzyme assays. By incorporating this reagent, scientists can isolate specific proteins for further study, contributing to advancements in understanding cellular functions. -
Question:
What are the dilution instructions for EMS 22146 Triton X-100?
Answer: Dilution instructions for EMS 22146 Triton X-100 depend on the specific application. Generally, a working concentration between 0.01% to 0.5% is recommended for various experiments. To prepare a dilution, mix the desired amount of the Triton X-100 solution with appropriate buffer or distilled water. Always verify the dilution requirements based on your experimental protocols, as precise concentrations can influence the outcomes significantly in biochemical assays. -
Question:
Is EMS 22146 Triton X-100 safe to handle?
Answer: While EMS 22146 Triton X-100 is generally safe when used properly, it is essential to handle it with care. It can be slightly irritating to skin and eyes; thus, wearing suitable personal protective equipment such as gloves and goggles is recommended during handling. Familiarize yourself with safety data sheets (SDS) for detailed safety information and appropriate disposal methods. By following these precautions, researchers can safely incorporate this surfactant into their laboratory protocols. -
Question:
What is the shelf life of EMS 22146 Triton X-100?
Answer: Typically, EMS 22146 Triton X-100 has a shelf life of up to 24 months when unopened and stored properly under recommended conditions. After opening, it's best to use the solution within 12 months for optimal performance. Always check for any changes in appearance or odor before use, and refer to storage recommendations on the label. Adhering to these guidelines helps ensure consistent results in experimental applications over time. -
Question:
Can EMS 22146 Triton X-100 be used for DNA extraction?
Answer: Yes, EMS 22146 Triton X-100 can be utilized in DNA extraction protocols. It serves as a gentle detergent that assists in lysing cells and breaking down cellular membranes without damaging nucleic acids. When preparing a lysis buffer, incorporating Triton X-100 can enhance the yield of purified DNA, making it suitable for downstream applications such as PCR, sequencing, or cloning. Always consider the specific requirements of your DNA extraction protocol for optimal results. -
Question:
What are the environmental impacts of using EMS 22146 Triton X-100?
Answer: EMS 22146 Triton X-100 is biodegradable but can have harmful effects on aquatic life if improperly disposed of in large quantities. It is crucial to dispose of any excess or waste according to local regulations, typically through appropriate hazardous waste disposal methods. Researchers should be mindful of the concentration and volume used in experiments and follow best practices to minimize environmental impact, thereby ensuring responsible usage in laboratory settings. -
Question:
Where can I buy EMS 22146 Triton X-100, 0.08% Aqueous Solution, 500 mL?
Answer: You can buy EMS 22146 Triton X-100, 0.08% Aqueous Solution, 500 mL from Ubuy. Ubuy offers a user-friendly shopping experience with a wide range of laboratory reagents, ensuring you find the product easily. Just visit the Ubuy website, search for the product, and place your order for quick access to this essential laboratory solution.
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UZS 1363300
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Ubuy works hard to protect your security and privacy. Our advanced payment security system ensures confidentiality by encrypting your information during transmission using AES (Advanced Encryption Standards) and SSL (Secure Socket Layer) protocols. Your payment details are 100% secure as we do not share your payment details with third party sellers.